75trust / 100

Micro Biology Qpcr Relative Expression

by qPCR Relative Expression in Other

x402 APIPassing, checked 42 min ago

Calculate a Pfaffl expression ratio from supplied Ct values and amplification efficiencies; require a declared stable reference. No diagnostic interpretation.

POST
https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression
Price
$0.058
per call in USDC
Uptime
100%
last 30 days
Response time
324 ms
415 ms slowest 5%
Last check
Passed
42 min ago, next any minute now

Run a free test callHow to call it

Reliability

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Status and outage history · Atom feed

How to call it

POST https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression

Each call costs $0.058001 in USDC on Base, paid with the request over x402.

Run a free test callOne unpaid request, the way an agent sends it. You see the payment challenge it gets back. Nothing is charged.

Example request

From the listing.

POST https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression
content-type: application/json

{
  "dataClass": "synthetic",
  "referenceControlCt": 18,
  "referenceEfficiency": 1,
  "referenceSampleCt": 18,
  "referenceStable": true,
  "targetControlCt": 22,
  "targetEfficiency": 1,
  "targetSampleCt": 20
}

Parameters

ParameterTypeRequiredDescription
dataClassstringYes One of: public, synthetic.
referenceControlCtnumberYes
referenceEfficiencynumberYes
referenceSampleCtnumberYes
referenceStablebooleanYes
targetControlCtnumberYes
targetEfficiencynumberYes
targetSampleCtnumberYes

Example response

The provider's example. We can't check it without paying.

{
  "amountBaseUnits": "58001",
  "boundaries": [
    "Public or synthetic inputs only. Caller labels are declarations,…"
  ],
  "inputDigest": "sha256:b3c2d52ef73e664ccdb18443e77c7892ada057676d13c750336c96338…",
  "offerId": "biology-qpcr-relative-expression",
  "receiptDigest": "sha256:0b1237155ed67a9c3a98f11700432739dd7c5f44557cbf58588386059…",
  "result": {
    "findings": {
      "deltaDeltaCt": -2,
      "expressionRatio": 4,
      "method": "Pfaffl ratio; efficiencies supplied as amplification increment (…",
      "referenceStabilityAssumed": true
    },
    "limitations": [
      "Public or synthetic research inputs only; no patient, personal g…"
    ],
    "methodVersion": "maha-science50/1.0.0",
    "numericPrecision": "IEEE754-double; finite outputs rounded to 12 significant digits"
  },
  "version": "maha-microproducts/0.1"
}

Call it from code

The curl command shows the payment challenge. The JavaScript and Python versions pay and call, and never pay more than $0.058001.

curl: see the payment challenge
# bash or zsh: see the payment challenge. Nothing is charged.
curl -sS -g -i -X POST 'https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression' \
  -H 'content-type: application/json' \
  --data-raw '{"dataClass":"synthetic","referenceControlCt":18,"referenceEfficiency":1,"referenceSampleCt":18,"referenceStable":true,"targetControlCt":22,"targetEfficiency":1,"targetSampleCt":20}'

# x402 v2 sends the challenge base64-encoded in the PAYMENT-REQUIRED header (v1 sends it in the body).
# To read the header:
curl -sS -g -o /dev/null -D - -X POST 'https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression' \
  -H 'content-type: application/json' \
  --data-raw '{"dataClass":"synthetic","referenceControlCt":18,"referenceEfficiency":1,"referenceSampleCt":18,"referenceStable":true,"targetControlCt":22,"targetEfficiency":1,"targetSampleCt":20}' \
  | grep -i '^payment-required:' | cut -d' ' -f2 | tr -d '\r' | base64 --decode
JavaScript: pay and call
// npm i @x402/fetch @x402/evm viem
import { wrapFetchWithPayment, x402Client } from "@x402/fetch";
import { registerExactEvmScheme } from "@x402/evm/exact/client";
import { privateKeyToAccount } from "viem/accounts";

// Pays at most $0.058001 per call.
const client = new x402Client().setSpendControls({ maxAmountPerPayment: "$0.058001" });
registerExactEvmScheme(client, { signer: privateKeyToAccount(process.env.EVM_PRIVATE_KEY) });
const pay = wrapFetchWithPayment(fetch, client);

// Not sure it's safe to pay? ToolVet checks it first for $0.005. Pay for that from a separate
// client: this one is capped at $0.058001 per payment. GET https://toolvet.app/api/v1/check?url=https%3A%2F%2Fwww.mahastrategies.com%2Fapi%2Fv1%2Fmicro%2Fbiology-qpcr-relative-expression&method=POST
const res = await pay("https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression", {
  method: "POST",
  headers: { "content-type": "application/json" },
  body: JSON.stringify({
    "dataClass": "synthetic",
    "referenceControlCt": 18,
    "referenceEfficiency": 1,
    "referenceSampleCt": 18,
    "referenceStable": true,
    "targetControlCt": 22,
    "targetEfficiency": 1,
    "targetSampleCt": 20
  }),
});
console.log(res.status, await res.text());
Python: pay and call
# pip install "x402[requests,evm]"
import os
from eth_account import Account
from x402 import x402ClientSync
from x402.http.clients import x402_requests
from x402.mechanisms.evm import EthAccountSigner
from x402.mechanisms.evm.exact.register import register_exact_evm_client

# Pays at most $0.058001 per call.
client = x402ClientSync().set_spend_controls({"max_amount_per_payment": "$0.058001"})
register_exact_evm_client(client, EthAccountSigner(Account.from_key(os.environ["EVM_PRIVATE_KEY"])))

with x402_requests(client) as session:
    res = session.post(
        "https://www.mahastrategies.com/api/v1/micro/biology-qpcr-relative-expression",
        json={
            "dataClass": "synthetic",
            "referenceControlCt": 18,
            "referenceEfficiency": 1,
            "referenceSampleCt": 18,
            "referenceStable": True,
            "targetControlCt": 22,
            "targetEfficiency": 1,
            "targetSampleCt": 20,
        },
    )
    print(res.status_code, res.text)

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Recent checks

WhenResultHTTPTimePrice
42 min agoPassed402317 ms$0.058
5 h agoPassed402353 ms$0.058
9 h agoPassed402394 ms$0.058
13 h agoPassed402415 ms$0.058
17 h agoPassed402242 ms$0.058
Show 10 older checks
21 h agoPassed402385 ms$0.058
25 h agoPassed402324 ms$0.058
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36 h agoPassed402326 ms$0.058
40 h agoPassed402274 ms$0.058
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47 h agoPassed402306 ms$0.058
2 days agoPassed402376 ms$0.058
2 days agoPassed402307 ms$0.058