75trust / 100

Micro Biology Qpcr Run Quality

by qPCR run-quality check in Other

x402 APIPassing, checked 4 h ago

Check supplied Ct replicates, efficiency and control declarations against fixed research QC bands; report Ct SD and failed conditions. No diagnostic validation.

POST
https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality
Price
$0.042
per call in USDC
Uptime
100%
last 30 days
Response time
328 ms
523 ms slowest 5%
Last check
Passed
4 h ago, next any minute now

Run a free test callHow to call it

Reliability

All checks passedSome failedAll failedNot checked

Status and outage history · Atom feed

How to call it

POST https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality

Each call costs $0.042001 in USDC on Base, paid with the request over x402.

Run a free test callOne unpaid request, the way an agent sends it. You see the payment challenge it gets back. Nothing is charged.

Example request

From the listing.

POST https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality
content-type: application/json

{
  "ct": [
    22,
    22.2,
    22.1
  ],
  "dataClass": "synthetic",
  "efficiency": 1,
  "meltSinglePeak": true,
  "noRTNegative": true,
  "noTemplateNegative": true,
  "referenceStable": true
}

Parameters

ParameterTypeRequiredDescription
ctarrayYes
dataClassstringYes One of: public, synthetic.
efficiencynumberYes
meltSinglePeakbooleanYes
noRTNegativebooleanYes
noTemplateNegativebooleanYes
referenceStablebooleanYes

Example response

The provider's example. We can't check it without paying.

{
  "amountBaseUnits": "42001",
  "boundaries": [
    "Public or synthetic inputs only. Caller labels are declarations,…"
  ],
  "inputDigest": "sha256:ce837e5ed4d4bd86f7a5c37959631499e2f0bae9ff126faaf3a1a0d1c…",
  "offerId": "biology-qpcr-run-quality",
  "receiptDigest": "sha256:31d6ef5a2a1bf3ba7d823ef71b06a935a42e396347ecc1bb8f5178a20…",
  "result": {
    "findings": {
      "assayValidated": false,
      "ctSD": 0.1,
      "declarationsComplete": true,
      "issues": [],
      "meanCt": 22.1
    },
    "limitations": [
      "Public or synthetic research inputs only; no patient, personal g…"
    ],
    "methodVersion": "maha-science50/1.0.0",
    "numericPrecision": "IEEE754-double; finite outputs rounded to 12 significant digits"
  },
  "version": "maha-microproducts/0.1"
}

Call it from code

The curl command shows the payment challenge. The JavaScript and Python versions pay and call, and never pay more than $0.042001.

curl: see the payment challenge
# bash or zsh: see the payment challenge. Nothing is charged.
curl -sS -g -i -X POST 'https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality' \
  -H 'content-type: application/json' \
  --data-raw '{"ct":[22,22.2,22.1],"dataClass":"synthetic","efficiency":1,"meltSinglePeak":true,"noRTNegative":true,"noTemplateNegative":true,"referenceStable":true}'

# x402 v2 sends the challenge base64-encoded in the PAYMENT-REQUIRED header (v1 sends it in the body).
# To read the header:
curl -sS -g -o /dev/null -D - -X POST 'https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality' \
  -H 'content-type: application/json' \
  --data-raw '{"ct":[22,22.2,22.1],"dataClass":"synthetic","efficiency":1,"meltSinglePeak":true,"noRTNegative":true,"noTemplateNegative":true,"referenceStable":true}' \
  | grep -i '^payment-required:' | cut -d' ' -f2 | tr -d '\r' | base64 --decode
JavaScript: pay and call
// npm i @x402/fetch @x402/evm viem
import { wrapFetchWithPayment, x402Client } from "@x402/fetch";
import { registerExactEvmScheme } from "@x402/evm/exact/client";
import { privateKeyToAccount } from "viem/accounts";

// Pays at most $0.042001 per call.
const client = new x402Client().setSpendControls({ maxAmountPerPayment: "$0.042001" });
registerExactEvmScheme(client, { signer: privateKeyToAccount(process.env.EVM_PRIVATE_KEY) });
const pay = wrapFetchWithPayment(fetch, client);

// Not sure it's safe to pay? ToolVet checks it first for $0.005. Pay for that from a separate
// client: this one is capped at $0.042001 per payment. GET https://toolvet.app/api/v1/check?url=https%3A%2F%2Fwww.mahastrategies.com%2Fapi%2Fv1%2Fmicro%2Fbiology-qpcr-run-quality&method=POST
const res = await pay("https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality", {
  method: "POST",
  headers: { "content-type": "application/json" },
  body: JSON.stringify({
    "ct": [
      22,
      22.2,
      22.1
    ],
    "dataClass": "synthetic",
    "efficiency": 1,
    "meltSinglePeak": true,
    "noRTNegative": true,
    "noTemplateNegative": true,
    "referenceStable": true
  }),
});
console.log(res.status, await res.text());
Python: pay and call
# pip install "x402[requests,evm]"
import os
from eth_account import Account
from x402 import x402ClientSync
from x402.http.clients import x402_requests
from x402.mechanisms.evm import EthAccountSigner
from x402.mechanisms.evm.exact.register import register_exact_evm_client

# Pays at most $0.042001 per call.
client = x402ClientSync().set_spend_controls({"max_amount_per_payment": "$0.042001"})
register_exact_evm_client(client, EthAccountSigner(Account.from_key(os.environ["EVM_PRIVATE_KEY"])))

with x402_requests(client) as session:
    res = session.post(
        "https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality",
        json={
            "ct": [
                22,
                22.2,
                22.1,
            ],
            "dataClass": "synthetic",
            "efficiency": 1,
            "meltSinglePeak": True,
            "noRTNegative": True,
            "noTemplateNegative": True,
            "referenceStable": True,
        },
    )
    print(res.status_code, res.text)

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