Micro Biology Qpcr Run Quality
by qPCR run-quality check in Other
Check supplied Ct replicates, efficiency and control declarations against fixed research QC bands; report Ct SD and failed conditions. No diagnostic validation.
https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality- Price
- $0.042
- per call in USDC
- Uptime
- 100%
- last 30 days
- Response time
- 328 ms
- 523 ms slowest 5%
- Last check
- Passed
- 4 h ago, next any minute now
Reliability
How to call it
POST https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality
Each call costs $0.042001 in USDC on Base, paid with the request over x402.
Run a free test callOne unpaid request, the way an agent sends it. You see the payment challenge it gets back. Nothing is charged.
Example request
From the listing.
POST https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality
content-type: application/json
{
"ct": [
22,
22.2,
22.1
],
"dataClass": "synthetic",
"efficiency": 1,
"meltSinglePeak": true,
"noRTNegative": true,
"noTemplateNegative": true,
"referenceStable": true
}Parameters
| Parameter | Type | Required | Description |
|---|---|---|---|
ct | array | Yes | |
dataClass | string | Yes | One of: public, synthetic. |
efficiency | number | Yes | |
meltSinglePeak | boolean | Yes | |
noRTNegative | boolean | Yes | |
noTemplateNegative | boolean | Yes | |
referenceStable | boolean | Yes |
Example response
The provider's example. We can't check it without paying.
{
"amountBaseUnits": "42001",
"boundaries": [
"Public or synthetic inputs only. Caller labels are declarations,…"
],
"inputDigest": "sha256:ce837e5ed4d4bd86f7a5c37959631499e2f0bae9ff126faaf3a1a0d1c…",
"offerId": "biology-qpcr-run-quality",
"receiptDigest": "sha256:31d6ef5a2a1bf3ba7d823ef71b06a935a42e396347ecc1bb8f5178a20…",
"result": {
"findings": {
"assayValidated": false,
"ctSD": 0.1,
"declarationsComplete": true,
"issues": [],
"meanCt": 22.1
},
"limitations": [
"Public or synthetic research inputs only; no patient, personal g…"
],
"methodVersion": "maha-science50/1.0.0",
"numericPrecision": "IEEE754-double; finite outputs rounded to 12 significant digits"
},
"version": "maha-microproducts/0.1"
}Call it from code
The curl command shows the payment challenge. The JavaScript and Python versions pay and call, and never pay more than $0.042001.
curl: see the payment challenge
# bash or zsh: see the payment challenge. Nothing is charged.
curl -sS -g -i -X POST 'https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality' \
-H 'content-type: application/json' \
--data-raw '{"ct":[22,22.2,22.1],"dataClass":"synthetic","efficiency":1,"meltSinglePeak":true,"noRTNegative":true,"noTemplateNegative":true,"referenceStable":true}'
# x402 v2 sends the challenge base64-encoded in the PAYMENT-REQUIRED header (v1 sends it in the body).
# To read the header:
curl -sS -g -o /dev/null -D - -X POST 'https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality' \
-H 'content-type: application/json' \
--data-raw '{"ct":[22,22.2,22.1],"dataClass":"synthetic","efficiency":1,"meltSinglePeak":true,"noRTNegative":true,"noTemplateNegative":true,"referenceStable":true}' \
| grep -i '^payment-required:' | cut -d' ' -f2 | tr -d '\r' | base64 --decodeJavaScript: pay and call
// npm i @x402/fetch @x402/evm viem
import { wrapFetchWithPayment, x402Client } from "@x402/fetch";
import { registerExactEvmScheme } from "@x402/evm/exact/client";
import { privateKeyToAccount } from "viem/accounts";
// Pays at most $0.042001 per call.
const client = new x402Client().setSpendControls({ maxAmountPerPayment: "$0.042001" });
registerExactEvmScheme(client, { signer: privateKeyToAccount(process.env.EVM_PRIVATE_KEY) });
const pay = wrapFetchWithPayment(fetch, client);
// Not sure it's safe to pay? ToolVet checks it first for $0.005. Pay for that from a separate
// client: this one is capped at $0.042001 per payment. GET https://toolvet.app/api/v1/check?url=https%3A%2F%2Fwww.mahastrategies.com%2Fapi%2Fv1%2Fmicro%2Fbiology-qpcr-run-quality&method=POST
const res = await pay("https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality", {
method: "POST",
headers: { "content-type": "application/json" },
body: JSON.stringify({
"ct": [
22,
22.2,
22.1
],
"dataClass": "synthetic",
"efficiency": 1,
"meltSinglePeak": true,
"noRTNegative": true,
"noTemplateNegative": true,
"referenceStable": true
}),
});
console.log(res.status, await res.text());Python: pay and call
# pip install "x402[requests,evm]"
import os
from eth_account import Account
from x402 import x402ClientSync
from x402.http.clients import x402_requests
from x402.mechanisms.evm import EthAccountSigner
from x402.mechanisms.evm.exact.register import register_exact_evm_client
# Pays at most $0.042001 per call.
client = x402ClientSync().set_spend_controls({"max_amount_per_payment": "$0.042001"})
register_exact_evm_client(client, EthAccountSigner(Account.from_key(os.environ["EVM_PRIVATE_KEY"])))
with x402_requests(client) as session:
res = session.post(
"https://www.mahastrategies.com/api/v1/micro/biology-qpcr-run-quality",
json={
"ct": [
22,
22.2,
22.1,
],
"dataClass": "synthetic",
"efficiency": 1,
"meltSinglePeak": True,
"noRTNegative": True,
"noTemplateNegative": True,
"referenceStable": True,
},
)
print(res.status_code, res.text)Security scan
No findings
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Recent checks
| When | Result | HTTP | Time | Price |
|---|---|---|---|---|
| 4 h ago | Passed | 402 | 255 ms | $0.042 |
| 8 h ago | Passed | 402 | 281 ms | $0.042 |
| 12 h ago | Passed | 402 | 364 ms | $0.042 |
| 16 h ago | Passed | 402 | 333 ms | $0.042 |
| 21 h ago | Passed | 402 | 362 ms | $0.042 |
Show 10 older checks
| 25 h ago | Passed | 402 | 270 ms | $0.042 |
| 28 h ago | Passed | 402 | 328 ms | $0.042 |
| 32 h ago | Passed | 402 | 279 ms | $0.042 |
| 36 h ago | Passed | 402 | 349 ms | $0.042 |
| 40 h ago | Passed | 402 | 290 ms | $0.042 |
| 44 h ago | Passed | 402 | 523 ms | $0.042 |
| 47 h ago | Passed | 402 | 372 ms | $0.042 |
| 2 days ago | Passed | 402 | 429 ms | $0.042 |
| 2 days ago | Passed | 402 | 329 ms | $0.042 |
| 2 days ago | Passed | 402 | 310 ms | $0.042 |